Alpha & Beta Glucans Test

Method Description

Alpha & Beta Glucans Test: Glucans are structural and storage polysaccharides composed of glucose units linked in alpha or beta configurations, with beta-glucans found predominantly in the cell walls of fungi, yeast, and cereals, and alpha-glucans serving as energy reserves in the form of glycogen and starch. Distinguishing between these two glucan types is important for manufacturers of mushroom-derived supplements, functional foods, and botanical extracts, because beta-glucan content is the biologically active fraction associated with immune-modulating properties and is the basis for potency claims, while alpha-glucan content reflects filler or storage carbohydrates that do not contribute to that activity. This enzymatic method selectively hydrolyzes alpha- and beta-glucan fractions using specific glucanase and amylase enzyme cocktails, quantifying each fraction independently so formulators and quality teams can verify label claims, assess raw material potency, and differentiate high-quality mushroom fruiting body extracts from myceliated grain products with elevated starch content.