Cholesterol
None
GC-FID
Method Description
This method determines cholesterol content in food samples. Lipids are released from the sample by acid or base hydrolysis and extracted into mixed ethers. Fatty acid-containing compounds are then interesterified to form fatty acid methyl esters (FAMEs) and free sterols. The components are separated and quantified by gas chromatography (GC) with flame ionization detection (FID). Cholestane is added prior to hydrolysis and used as an internal standard for quantitation.
Analyte List
1
analyte
| Analyte Name | LOD | LOQ | LCL |
|---|---|---|---|
| Cholesterol | N/A | N/A | N/A |
Technical Data Sheet
Test Name (LIMS/CoA)
Cholesterol - GC-FID
Method Name
Cholesterol Test
Platform Type
GC-FID
Platform Details
Gas Chromatograph with Flame Ionization Detection
Result Type
Quantitative - Single Analyte
Reportable Unit
mg/g
Method References
AOAC OMA 976.26
Acceptable Matrices
Food and Food Ingredients
Unacceptable Matrices
Concentrates of Vitamin E or forms of Vitamin E such as tocopherols, tocopheryl succinate, tocotrienols, and gums.
*Prices and offerings are subject to change without notice.
Additional information
Amount Needed: 5 g
Cholesterol
qPCR
Test Code
GM006
Status
Active
TaT
1-2 Business Days
Performing Lab
Medallion Labs
Sample Needed
5 g
Accreditation
ISO 17025
References
AOAC
Category
Nutritional & Proximate
Subcategory
Lipids & Cholesterol
Industries Available
Dietary Supplements
Food
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