Fat Profile

Method Description

This method provides a breakdown of fat as Total Fat, Saturated Fat, Monounsaturated Fat, Polyunsaturated Fat, and Trans Fat. The method assumes fat is present primarily as triglycerides and calculates total fat from measured fatty acid methyl ester (FAME) values with an assumed glycerol backbone. As the method is designed to measure triglyceride content, accuracy may be affected in samples containing significant levels of monoglycerides, diglycerides, or phospholipids. Lipids are extracted from food samples using conventional Mojonnier techniques. The extracted lipids are then transesterified to form FAMEs, which are quantified using capillary gas chromatography (GC).