Moisture Content - Karl Fischer Test



Turnaround Time:

2-3 Days


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Moisture Content - Karl Fischer Test

Method Description

Moisture by Karl Fischer is determined by automated potentiometric titration with an iodine and sulfur dioxide reagent. Moisture is extracted into an appropriate solvent and is titrated volumetrically (as opposed to colorimetric titration). This method is applicable to high-fat samples or samples that contain compounds that may volatilize when heated or subjected to vacuum (e.g. alcohol, glycerine, etc.)

Analyte List

Analyte Name LOD LOQ
Moisture N/A N/A

Technical Data Sheet

Test Name - Common
Moisture Content - Karl Fischer Test
Method Name
Karl Fischer Coulometric Titration
Method Code
Standard titration setup
Method Reference
AOAC 984.20, USP <921>
Reportable Unit
Sample Size Requirements
20 grams
Available for
Food Products, Dietary Supplements, Botanicals (Raw Materials, Extracts, Finished Products), API (Isolates, Active Ingredients) Cannabis & Hemp (Extracts, Isolates, MIPs)
Additional Information
Moisture in most food materials can be determined by loss on drying using a Gravimetric Moisture method. For samples high in fat or that contain components that will volatilize under the conditions of the Gravimetric Moisture assays, the Karl Fischer titration may be more appropriate. Note that chemically bound water molecules may be incompletely detected by this method.
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