Phytic Acid
Enzymatic Assay
Method Description
This method determines phytic acid content in food samples. Phytic acid is extracted under acidic conditions and enzymatically hydrolyzed using phytase and phosphatase to release phosphorus. The released phosphorus is quantified colorimetrically using ammonium molybdate. Free (inorganic) phosphorus is measured separately and subtracted from the total to determine phosphorus derived from phytic acid. Phytic acid content is then calculated from the released phosphorus. Accurate sample descriptions or estimated phytic acid levels are recommended to support appropriate analysis.
Analyte List
| Analyte Name | LOD | LOQ | LCL |
|---|---|---|---|
| Phytic Acid | N/A | N/A | N/A |
Technical Data Sheet
Information required by submitted
Amount needed: 20 g.
Additional information
Performed by our partner laboratory (Medallion Labs, General Mills). Sample size and turnaround follow the partner’s requirements; the certificate of analysis is issued by Cambium Analytica. Phytic acid is extracted under acid, dephosphorylated with phytase and alkaline phosphatase, and the released phosphate is measured colorimetrically; partner LOQ 0.20 g/100 g.
Phytic Acid
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