Steviol Glycosides - Finished Goods - USP-FCC

Method Description

Steviol glycosides are naturally occurring sweet-tasting compounds — including rebaudioside A, stevioside, and several related glycosides — extracted from the leaves of Stevia rebaudiana, a South American plant widely used as a high-intensity, zero-calorie sweetener in foods, beverages, and dietary supplements. Quantifying individual steviol glycosides is essential for label accuracy, ingredient identity confirmation, and regulatory compliance, as FDA has authorized specific glycosides as Generally Recognized as Safe (GRAS) while the FCC establishes purity and composition standards for finished products. This method employs HPLC with diode array detection against authenticated reference standards per the FCC Steviol Glycosides Monograph, resolving and quantifying individual glycoside peaks in finished matrices including powdered beverage mixes, functional foods, and dietary supplements.