Steviol Glycosides - Ingredient - USP-FCC

STEVG

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Turnaround Time:

2-5 business days

Steviol Glycosides - Ingredient - USP-FCC

Method Description

Steviol glycosides are the naturally derived sweet-tasting compounds found in the leaves of Stevia rebaudiana, encompassing a family of related diterpene glycosides — including stevioside, rebaudioside A, rebaudioside D, and others — that deliver intense sweetness without caloric contribution. Quantifying individual and total steviol glycoside content is central to ingredient specification compliance, label accuracy for sweetener systems, and meeting FCC and FDA identity and purity standards for food-grade stevia extracts. This method employs HPLC with diode array detection against authenticated reference standards to separate, identify, and quantify the full steviol glycoside profile in stevia leaf extracts, purified preparations, and rebaudioside or stevioside ingredients, as defined in the FCC Steviol Glycosides Monograph.

Analyte List

Analyte NameLODLOQ
Dulcoside A0.002 mg/g0.005 mg/g
Dulcoside A (Dry Weight Basis)0.002 mg/g0.005 mg/g
Rebaudioside A0.002 mg/g0.004 mg/g
Rebaudioside A (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside B0.002 mg/g0.004 mg/g
Rebaudioside B (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside C0.002 mg/g0.004 mg/g
Rebaudioside C (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside D0.002 mg/g0.004 mg/g
Rebaudioside D (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside E0.001 mg/g0.002 mg/g
Rebaudioside E (Dry Weight Basis)0.001 mg/g0.002 mg/g
Rebaudioside F0.002 mg/g0.004 mg/g
Rebaudioside F (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside M0.002 mg/g0.004 mg/g
Rebaudioside M (Dry Weight Basis)0.002 mg/g0.004 mg/g
Rebaudioside N0.001 mg/g0.002 mg/g
Rebaudioside N (Dry Weight Basis)0.001 mg/g0.002 mg/g
Rebaudioside O0.001 mg/g0.002 mg/g
Rebaudioside O (Dry Weight Basis)0.001 mg/g0.002 mg/g
Rubusoside0.002 mg/g0.004 mg/g
Rubusoside (Dry Weight Basis)0.002 mg/g0.004 mg/g
Steviolbioside0.002 mg/g0.004 mg/g
Steviolbioside (Dry Weight Basis)0.002 mg/g0.004 mg/g
Stevioside0.002 mg/g0.004 mg/g
Stevioside (Dry Weight Basis)0.002 mg/g0.004 mg/g
Total Steviol GlycosidesN/AN/A
Total Steviol Glycosides (Dry Weight Basis)N/AN/A

Technical Data Sheet

Method Name
Determination of Steviol Glycosides by HPLC-UV
Method Code
ANA-TM-127
Equipment Type
Ultra High Performance Liquid Chromatography - Diode-Array Detection (UPLC-DAD)
Equipment Details
Method Reference
FCC Steviol Glycosides Monograph, HPLC Assay
Reportable Unit
%, mg/g
Sample Size Requirements
10 g
Available for
Steviol Glycoside Ingredients; Stevia Leaf Extracts; Purified Steviol Glycoside Preparations; Rebaudioside Ingredients; Stevioside Ingredients; Steviol Glycoside Sweetener Ingredients
Additional Information
Must be ordered with Equilibrated Moisture Content (MOSEQ).
Unavailable for
Finished dietary supplements; Ready-to-drink beverages; Powdered beverage mixes; Gummies, capsules, tablets, softgels, or other finished dosage forms; Products where steviol glycosides are only one component of the finished formulation (order STEVA instead)
*Prices and offerings are subject to change without notice.

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