Bacillus cereus
bioMérieux BACARA® Agar
Method Description
Bacillus cereus Suitability Testing confirms that the method can effectively detect and recover B. cereus in a specific sample matrix. The assessment includes analysis of a fortified sample (matrix spike) and a laboratory control sample (LCS) prepared in a contaminant-free matrix. Recovery from the sample is compared to the LCS to evaluate matrix effects and ensure reliable detection. The Method utilizes selective and differential media to isolate B. cereus based on characteristic colony morphology and selective and differential characteristics.
Analyte List
| Analyte Name | LOD | LOQ | LCL |
|---|---|---|---|
| Bacillus Cereus (Laboratory Control Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Bacillus Cereus (Matrix Spike) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Bacillus Cereus (Product Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Bacillus Cereus (Method Suitability - Relative Recovery) | N/A | N/A | N/A |
Technical Data Sheet
Additional information
Product-specific suitability per USP <2021> (suitability of the counting method in the presence of the product). Three preparations are analysed side by side: the Product Sample as received, a Matrix Spike (the product inoculated with a low, known level of the target organism, not more than 100 CFU) and a Laboratory Control Sample (the same inoculum in diluent, without product). Relative Recovery (%) = Matrix Spike count ÷ Laboratory Control Sample count, after subtracting any background in the Product Sample. The reportable range 50 – 200% is the USP acceptance criterion (recovery within a factor of two): a result inside the range shows the product neither inhibits nor enhances the method, and routine USP-referenced testing of that product can proceed. Outside the range the method is modified (neutralisers, further dilution or membrane filtration) and the suitability is repeated.
Bacillus cereus
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