Total Yeast & Mold
Neogen® Petrifilm® Rapid Yeast and Mold Count Plate
Method Description
Total Yeast and Mold Suitability Testing confirms that the Method can effectively detect and recover yeast and mold in a specific sample matrix. The assessment includes analysis of a fortified sample (matrix spike) and a laboratory control sample (LCS). Recovery from the sample is compared to the LCS to evaluate matrix effects and ensure reliable detection across a broad range of fungal concentrations.
Analyte List
| Analyte Name | LOD | LOQ | LCL |
|---|---|---|---|
| Total Mold (Laboratory Control Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Mold (Matrix Spike) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Mold (Product Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Yeast (Laboratory Control Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Yeast (Matrix Spike) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Yeast (Product Sample) | 1.000 CFU/g | 1.000 CFU/g | N/A |
| Total Mold (Method Suitability - Relative Recovery) | N/A | N/A | N/A |
| Total Yeast (Method Suitability - Relative Recovery) | N/A | N/A | N/A |
Technical Data Sheet
Additional information
Product-specific suitability per USP <2021> (suitability of the counting method in the presence of the product). Three preparations are analysed side by side: the Product Sample as received, a Matrix Spike (the product inoculated with a low, known level of the target organism, not more than 100 CFU) and a Laboratory Control Sample (the same inoculum in diluent, without product). Relative Recovery (%) = Matrix Spike count ÷ Laboratory Control Sample count, after subtracting any background in the Product Sample. The reportable range 50 – 200% is the USP acceptance criterion (recovery within a factor of two): a result inside the range shows the product neither inhibits nor enhances the method, and routine USP-referenced testing of that product can proceed. Outside the range the method is modified (neutralisers, further dilution or membrane filtration) and the suitability is repeated.
Total Yeast & Mold
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