Vitamin B1
None
Thiamine
UPLC-DAD
Method Description
Thiamine (vitamin B1) is quantified by UPLC with diode-array detection and reported as thiamine, with thiamine hydrochloride and thiamine mononitrate equivalents calculated for label-claim comparison. Used for fortified foods and dietary supplements to confirm declared content and support nutrition labeling.
Analyte List
3
analytes
| Analyte Name | CAS # | Type |
|---|---|---|
| Vitamin B1 (Thiamine) | 70-16-6 | Measured |
| Vitamin B1 as Thiamine HCl | 67-03-8 | Total (Sum) |
| Vitamin B1 as Thiamine Monohydrate | 5947-14-6 | Total (Sum) |
Technical Data Sheet
Test Name (LIMS/CoA)
Vitamin B1 (Thiamine) - UPLC-DAD
Method Name
Determination of Vitamin B1, C, and B3 by UPLC-DAD
Method Code
ANA-TM-093
Platform Type
UPLC-DAD
Platform Details
UPLC with diode-array detection
Result Type
Calculated
Method References
USP–NF Dietary Supplement monograph: Water-Soluble Vitamins Tablets (Assay procedures)
Reference Modifications
Modified method — UPLC-DAD in place of the monograph HPLC procedure; extended to non-tablet matrices; thiamine mononitrate equivalent calculated
Limit of Quantitation
Dynamic Reporting Limit: The reported LOD and LOQ
Acceptable Matrices
Food Products, Dietary Supplements
*Prices and offerings are subject to change without notice.
Information required by submitted
Please provide the expected target value(s) or label claim(s) and the serving size in the sample notes when submitting your order, if available.
Additional information
Reported as % (w/w) and mg/g; label-claim comparison is available when the serving size is supplied.
Vitamin B1
Thiamine
UPLC-DAD
Test Code
VITB1
Status
Active
TaT
1-2 Business Days
Performing Lab
Cambium Analytica - In-House
Sample Needed
20 g
Accreditation
ISO 17025
References
USP
Cambium Analytica
Category
Vitamin
Subcategory
Water-Soluble Vitamins
Industries Available
Dietary Supplements
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