Vitamin B1 (Thiamine) Test



Turnaround Time:

2-3 Days

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Vitamin B1 (Thiamine) Test

Method Description

The determination of water-soluble vitamins in food and dietary supplements using Ultra-Performance Liquid Chromatography with Diode Array Detection (UPLC-DAD) involves several steps. Firstly, sample preparation is carried out to extract the water-soluble vitamins from the matrix. Then, the extracted samples are injected into the UPLC system equipped with a Diode Array Detector (DAD). The UPLC separates the individual vitamins based on their chemical properties and elution times. The DAD detects the presence of the vitamins by measuring their absorbance at specific wavelengths. Calibration curves prepared using standard solutions allow for quantification of the vitamins in the samples. This method offers high sensitivity, precision, and efficiency for analyzing water-soluble vitamins in complex matrices.

Analyte List

Analyte Name LOD LOQ
Vitamin B1 (Thiamine) N/A N/A

Technical Data Sheet

Test Name - Common
Vitamin B1 (Thiamine) Test
Method Name
Determination of Vitamin B1, C, and B3 by UHPLC/DAD
Method Code
Ultra-Performance Liquid Chromatography with Diode-Array Detection (UPLC-DAD)
Method Reference
Cambium Analytica Developed
Reportable Unit
%, mg/g
Sample Size Requirements
20 grams
Available for
Food Products, Dietary Supplements
Additional Information
*Prices and offerings are subject to change without notice.