Vitamin B12
None
Cyanocobalamin & Methylcobalamin
UPLC-DAD
Method Description
Cyanocobalamin and methylcobalamin, the two common supplemental forms of vitamin B12, are quantified individually by UPLC with diode-array detection for dietary supplements and fortified foods to confirm the declared form and content.
Analyte List
2
analytes
| Analyte Name | CAS # | Type |
|---|---|---|
| Vitamin B12 (Cyanocobalamin) | 68-19-9 | Measured |
| Vitamin B12 (Methylcobalamin) | 13422-55-4 | Measured |
Technical Data Sheet
Test Name (LIMS/CoA)
Vitamin B12 (Cyanocobalamin, Methylcobalamin) - UPLC-DAD
Method Name
Determination of Vitamin B12 by UPLC-DAD
Method Code
ANA-TM-097
Platform Type
UPLC-DAD
Platform Details
UPLC with diode-array detection
Result Type
Calculated
Method References
USP–NF Dietary Supplement monograph: Water-Soluble Vitamins Tablets (Assay procedures)
Reference Modifications
Modified method — UPLC-DAD in place of the monograph HPLC procedure; extended to non-tablet matrices; methylcobalamin is an in-house extension not covered by the monograph
Limit of Quantitation
Dynamic Reporting Limit: The reported LOD and LOQ
Acceptable Matrices
Food Products, Dietary Supplements
*Prices and offerings are subject to change without notice.
Information required by submitted
Please provide the expected target value(s) or label claim(s) and the serving size in the sample notes when submitting your order, if available.
Additional information
Reported as % (w/w) and mg/g; label-claim comparison is available when the serving size is supplied.
Vitamin B12
Cyanocobalamin & Methylcobalamin
UPLC-DAD
Test Code
VTB12
Status
Active
TaT
1-2 Business Days
Performing Lab
Cambium Analytica - In-House
Sample Needed
20 g
Accreditation
ISO 17025
References
USP
Cambium Analytica
Category
Vitamin
Subcategory
Water-Soluble Vitamins
Industries Available
Dietary Supplements
Food
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